SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. SpectraMax® ABS Plus. Here the delta mP was 336, and Z’ factor was 0. . Selection of Water Constant in PathCheck settings. Examples: “Press carefully to engage,” “ Do not. 2. Molecular Devices. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Small, unbound molecules rotate rapidly in. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Figure 1. 5 87. Keywords. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). TR-FRET assay. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. begin reading microplates with seven mouse clicks. Ltd. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. FlexStation® 3 Multi-Mode Microplate Reader. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. When the user applies a set temperature of 37°C, the top is heated to 37. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. #M5) SpectraMax® L Microplate Reader (cat. Key applications: ELISA, nucleic acid, endotoxin testing. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax® ABS. Bookings are free, but you will need to use iLab to book. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 59 6. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. Lift up the lid over the chamber to insert or remove a cuvette. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. TNF standard curve. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. book Page 7 Wednesday, July 7, 2010 2:09 PM. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. Or scan up to 384 wells at any time to determine the best assay conditions. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. Molecular Devices SpectraMax® series microplate readers. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 05 3. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Supersedes 1. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Maximum signal with lowest background. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Thank you!. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. 5 Dual-luciferase reporter assay. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Shop now. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Wide range of applications. 7°C and the bottom to 36. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. es •. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. com. p. Molecular Devices SpectraMax® series microplate readers. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. When a. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. When the user applies a set temperature of 37°C, the top is heated to 37. The integration time suggested is 5-10 sec. B: Selection of Excitation Wavelength. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Five preferred detection modes. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. When a feature or capability applies to only certain readers, this exception is noted. 43 Max %CV 1. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Fluorescence Microplate Reader Comparison Chart. 0. The guide also covers the software features, the specifications, and the accessories of the systems. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. Microplate Readers Biotek Epoch Microplate. Step 1. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. analysis, controls the SpectraMax Multi-Mode Microplate Readers. The plate reader can measure multiple readouts, including UV and visible absorbance. , Sunnyvale, CA) using the bottom well-scan mode where nine readings. E Models. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. SpectraMax® Microplate Readers and SoftMax® Pro Software. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. The dual monochromator optics allow the widest range of applications to be utilized for bior. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email [email protected]% Triton X‐100, 10 mM potassium phosphate buffer pH 7. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Molecular Devices. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Add to Cart. A: Microplate Preparation. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. When a feature or capability applies to only certain readers, this exception is noted. . The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Compact and tunable absorbance microplate readers DATA SHEET. • Tel. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). 1. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. Western blot analyses. com A. Published Dec 16, 2020 | Updated Dec 16, 2020. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Sale price $18,499. 1. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The acceptance. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 1. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. edu) to be added as a trained user in iLab. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. TNF standard curve. Compatible automation solutions for SpectraMax Readers. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. es •. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. The SoftMax® Touch. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. Incubation. Levels of immunoreactivity were normalized to. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. 4. 0 and 0. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. 2. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. ) by nonlinear regression using. Molecular Devices. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. Figure 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. 0 out of 5 $ 24,695. Shop now. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Table of Contents. Molecular Devices Spectramax M5 Microplate Reader; Related products. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 00. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Lower limits of detection for ATP determined using the ATPlite . The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. SpectraMax® M5. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Three independent experiments were performed. However, when a specific probe is not available, or it causes off-target effects on the. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. Self Service Available. Page 28 Chapter 5: Read Modes and Read Types 1. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. Add to Cart. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 303 unable to cal dark current. Expanded wavelength coverage. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. free tracer decreases, resulting in an. B: Selection of Excitation Wavelength. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. spectramax m5 multi. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. No accessories are needed and SoftMax® Pro Software. The following document is intended to demonstrate setup of this instrument. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). After 24 h drug treatment, cells were lysed by RIPA buffer (Life Technologies Inc, US). Sale price $16,495. 1. 0 μL) instead of the enzyme solution was used as the sample blank group. Cables. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. e. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Microbiota DNA was. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. The system. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. 95. view more . Table of Contents. Linear, Orbital Slow, and Orbital Fast. Note: In this user guide, SpectraMax ® refers to several SpectraMax . SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Fluorescence Microplate Readers. The dual monochromator optics allow the widest range of applications to be. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. 4. AquaMax® 4000. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. In brief, RAW 264. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Published Dec 16, 2020 | Updated Dec 16, 2020. Detailed specifications of each model reader are reported in Section 7, “Specifications”. SpectraMax M Series multi-mode microplates are configurable readers with triple. 315 can't find zero order. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Five preferred modes for diverse bioresearch applications. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Figure 1. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Catalog Number: (89212-396) Supplier: Molecular Devices. 00 USD. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. These robust readers have been placed in labs from Antarctica to. Do not touch the clear surfaces of the cuvette. Go to Product Details. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. SpectraMax M5e Multi-Mode Microplate Reader. Maximum signal with lowest background. Turn on the microplate reader. The Spectr. Choose from a. We would like to show you a description here but the site won’t allow us. FlexStation™ II. This application note describes how to. 63 1. KEY FEATURES • Five. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. SpectraMax® ABS. Figure 1. When a feature or capability applies to only certain readers, this exception is noted. The spectrophotometric. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Microplate Readers include the M3, M4,. Most papers they cultured bacteria to OD600>0. Category 2 and 3 microplate readers use gradient heating as well. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Depending on which. Molecular Devices SpectraMax M5 Multimode Microplate Reader. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 3 mg. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. ATP standard curves read on SpectraMax microplate readers. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. The SpectraMax M2 and M2e are versatile and reliable. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 1. Choose from a three- (M3), four-. 14 Average %CV 0. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Other technologies such as imaging. You can use either. 2. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Fluorescence Microplate Readers. ® reader to the computer. The cells were then treated to induce apoptosis. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 72 MB. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. microplate reader. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. flexibility of monochromator-based systems. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. book Page 7 Wednesday, July 7, 2010 2:09 PM. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. 3 Version 7. 0 out of 5 $ 16,499. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. book Page 7 Wednesday, July 7, 2010 2:09 PM. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. 1. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. All associated StakMax® stackers. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 67. M3-M5e_manual. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Flexible for 6-well to 384-well microplates and cuvettes. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 87. com 13. Plasma Tau was determined by high-performance liquid chromatography (Shimadzu ®, model LC 10AD) using a Shimadzu model RF 535 fluorescence detector. M3-M5e_manual.